Review



rabbit anti tph2  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Novus Biologicals rabbit anti tph2
    Rabbit Anti Tph2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/Tryptophan+hydroxylase+2+Antibody+-+BSA+Free/pmc13076638-372-10-13
    Average 95 stars, based on 63 article reviews
    rabbit anti tph2 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Habenular μ-opioid receptor knockout and chronic systemic receptor blockade promote negative affect and heighten nociceptive sensitivity
    Article Snippet: .. Following a 10-minute PBS wash, sections were incubated with primary antibodies—rabbit anti-MOR (1:300, abcam, ab134054) rabbit anti-TPH2 (1:1000, Novus Biologicals, NB100-74555), mouse anti-TH (1:1000, Millipore, MAB318), rat anti-mCherry (1:1000, Millipore, MAB131873), or chicken anti-GFP (1:1000, Aves Labs, GFP-1010)—in a carrier solution containing 0.5% Triton X-100 and 5% normal goat serum in PBS. ..

    Article Title: Alterations in gut microbiota and metabolites contribute to postoperative sleep disturbances.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense transferred onto polyvinylidene fluoride membranes, and blocked for 1 h. Then, the membranes were sequentially incubated with specific primary antibodies—rabbit anti- TPH1 (1:1000, Novus, cat. no. NBP267580), rabbit anti- TPH2 (1:1000, Novus, cat. no. NB100- 74555), rabbit anti- occludin (1:1000, Abcam, cat. no. ab216327), rabbit anti- BDNF (1:2000, Abcam, cat. no. ab108319), and rabbit anti- GAPDH (1:5000, Proteintech, cat. no. 10494- 1- AP)—overnight at 4°C and then with a goat anti- rabbit immunoglobulin G (IgG) horseradish peroxidase–conjugated antibody (1:10 000, Abcam, cat. no. ab6721) for 1 h at room temperature. .. Finally, the target proteins were treated with enhanced chemiluminescence solution and observed using a ChemiDoc MP Imaging System (Bio- Rad, USA).

    Article Title: Alterations in gut microbiota and metabolites contribute to postoperative sleep disturbances
    Article Snippet: .. Proteins were separated using 8%–12% gel electrophoresis, transferred onto polyvinylidene fluoride membranes, and blocked for 1 h. Then, the membranes were sequentially incubated with specific primary antibodies—rabbit anti‐TPH1 (1:1000, Novus, cat. no. NBP2‐67580), rabbit anti‐TPH2 (1:1000, Novus, cat. no. NB100‐74555), rabbit anti‐occludin (1:1000, Abcam, cat. no. ab216327), rabbit anti‐BDNF (1:2000, Abcam, cat. no. ab108319), and rabbit anti‐GAPDH (1:5000, Proteintech, cat. no. 10494‐1‐AP)—overnight at 4°C and then with a goat anti‐rabbit immunoglobulin G (IgG) horseradish peroxidase–conjugated antibody (1:10 000, Abcam, cat. no. ab6721) for 1 h at room temperature. .. Finally, the target proteins were treated with enhanced chemiluminescence solution and observed using a ChemiDoc MP Imaging System (Bio‐Rad, USA).

    Immunostaining:

    Article Title: Human neuromodulatory assembloids to study serotonin signaling and disease
    Article Snippet: Sections were washed once more with DPBS and cover glass was mounted overnight using Aqua-Poly/Mount (Polysciences, 18606). .. The following antibodies were used for immunostaining: anti-TPH2 antibody (1:500 dilution, rabbit, Novus Biologicals, NB100-74555), anti-5-HT antibody (1:1000 dilution, rabbit, Immunostar, 20080), and anti-GFP antibody (1:1000 dilution, rabbit, Invitrogen, A-21311). .. Immunostained sections were imaged on an inverted confocal microscope (Leica Stellaris 5).

    Labeling:

    Article Title: Hyperexcitability of female serotonin neurons underlies sex-specific anxiety responses
    Article Snippet: .. Coronal sections were cut (35 μm) on a vibratome (Leica VT1200) and labeled for eYFP and Tph2 using the following primary and secondary antibodies: anti-GFP in goat (1:5000; #NB100-1770, Novus Biologicals), anti-Tph2 in rabbit (1:2000; #NB100-74555, Novus Biological), anti-goat-A488 in donkey (1:2000; #A11055, Life Technologies) and anti-rabbit-A555 in donkey (1:2000; #A31572, Life Technologies). ..

    Nucleic Acid Electrophoresis:

    Article Title: Alterations in gut microbiota and metabolites contribute to postoperative sleep disturbances
    Article Snippet: .. Proteins were separated using 8%–12% gel electrophoresis, transferred onto polyvinylidene fluoride membranes, and blocked for 1 h. Then, the membranes were sequentially incubated with specific primary antibodies—rabbit anti‐TPH1 (1:1000, Novus, cat. no. NBP2‐67580), rabbit anti‐TPH2 (1:1000, Novus, cat. no. NB100‐74555), rabbit anti‐occludin (1:1000, Abcam, cat. no. ab216327), rabbit anti‐BDNF (1:2000, Abcam, cat. no. ab108319), and rabbit anti‐GAPDH (1:5000, Proteintech, cat. no. 10494‐1‐AP)—overnight at 4°C and then with a goat anti‐rabbit immunoglobulin G (IgG) horseradish peroxidase–conjugated antibody (1:10 000, Abcam, cat. no. ab6721) for 1 h at room temperature. .. Finally, the target proteins were treated with enhanced chemiluminescence solution and observed using a ChemiDoc MP Imaging System (Bio‐Rad, USA).

    Blocking Assay:

    Article Title: Serotonergic neurons in the dorsal raphe nucleus scale O₂ utilization in response to traumatic stress
    Article Snippet: A freezing microtome (CM1950; Leica Microsystems) was used to cut 40-μm-thick coronal sections. .. Free-floating sections were washed in PBS three times, 5 min/wash, permeabilized with 0.3% Triton X-100 in PBS for 30 min, blocked in blocking solution containing 5% normal goat serum and 0.3% Triton X-100 for 2 h at room temperature, and then stained with a rabbit polyclonal anti-TPH2 primary antibody (1:300, NB100-74555; Novus Biologicals) overnight at 4°C. .. After washing, the sections were incubated with the secondary antibody (1:500, Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L); Thermo Fisher Scientific) for 2 h, and mounted with a mounting medium containing diamidino-phenylindole (DAPI) (Vectashield; Vector Laboratories, Burlingame, CA, USA).

    Staining:

    Article Title: Serotonergic neurons in the dorsal raphe nucleus scale O₂ utilization in response to traumatic stress
    Article Snippet: A freezing microtome (CM1950; Leica Microsystems) was used to cut 40-μm-thick coronal sections. .. Free-floating sections were washed in PBS three times, 5 min/wash, permeabilized with 0.3% Triton X-100 in PBS for 30 min, blocked in blocking solution containing 5% normal goat serum and 0.3% Triton X-100 for 2 h at room temperature, and then stained with a rabbit polyclonal anti-TPH2 primary antibody (1:300, NB100-74555; Novus Biologicals) overnight at 4°C. .. After washing, the sections were incubated with the secondary antibody (1:500, Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L); Thermo Fisher Scientific) for 2 h, and mounted with a mounting medium containing diamidino-phenylindole (DAPI) (Vectashield; Vector Laboratories, Burlingame, CA, USA).



    Similar Products

    rabbit anti-tph2 antibody  (Thermo Fisher)
    90
    Thermo Fisher rabbit anti-tph2 antibody
    Rabbit Anti Tph2 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/rabbit+anti+tph2/pm40081797-82-6-9
    Average 90 stars, based on 1 article reviews
    rabbit anti-tph2 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    rabbit anti tph2  (Novus Biologicals)
    95
    Novus Biologicals rabbit anti tph2
    Rabbit Anti Tph2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/Tryptophan+hydroxylase+2+Antibody+-+BSA+Free/pmc13076638-372-10-13
    Average 95 stars, based on 1 article reviews
    rabbit anti tph2 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti tph2 primary antibody  (Novus Biologicals)
    95
    Novus Biologicals rabbit polyclonal anti tph2 primary antibody
    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, <t>anti-TPH2</t> antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.
    Rabbit Polyclonal Anti Tph2 Primary Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/Tryptophan+hydroxylase+2+Antibody+-+BSA+Free/bio_rxiv__64898__2026__01__14__699586-289-44-51
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal anti tph2 primary antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti tph2 in rabbit  (Novus Biologicals)
    95
    Novus Biologicals anti tph2 in rabbit
    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, <t>anti-TPH2</t> antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.
    Anti Tph2 In Rabbit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/Tryptophan+hydroxylase+2+Antibody+-+BSA+Free/bio_rxiv__2025__08__17__670715-307-31-36
    Average 95 stars, based on 1 article reviews
    anti tph2 in rabbit - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit anti tph2 antibody  (Novus Biologicals)
    95
    Novus Biologicals rabbit anti tph2 antibody
    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, <t>anti-TPH2</t> antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.
    Rabbit Anti Tph2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/Tryptophan+hydroxylase+2+Antibody+-+BSA+Free/pm39924929-153-16-21
    Average 95 stars, based on 1 article reviews
    rabbit anti tph2 antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    sc 374376 rabbit anti tph2 polyclonal antibody  (Santa Cruz Biotechnology)
    95
    Santa Cruz Biotechnology sc 374376 rabbit anti tph2 polyclonal antibody
    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, <t>anti-TPH2</t> antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.
    Sc 374376 Rabbit Anti Tph2 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-+tph2+antibody/HNF-3%CE%B2+Antibody/pmc11581877__mmc1-50-17-15
    Average 95 stars, based on 1 article reviews
    sc 374376 rabbit anti tph2 polyclonal antibody - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, anti-TPH2 antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.

    Journal: bioRxiv

    Article Title: Serotonergic neurons in the dorsal raphe nucleus scale O₂ utilization in response to traumatic stress

    doi: 10.64898/2026.01.14.699586

    Figure Lengend Snippet: a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, anti-TPH2 antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.

    Article Snippet: Free-floating sections were washed in PBS three times, 5 min/wash, permeabilized with 0.3% Triton X-100 in PBS for 30 min, blocked in blocking solution containing 5% normal goat serum and 0.3% Triton X-100 for 2 h at room temperature, and then stained with a rabbit polyclonal anti-TPH2 primary antibody (1:300, NB100-74555; Novus Biologicals) overnight at 4°C.

    Techniques: Labeling, Injection